International researchers of the Replica project from Italy, Greece, Oman, the USA and Serbia aimed to replicate three major international studies, testing them independently.
Exposure protocols for tests comply with international standards (ISO, CORESTA and HCI), and experimental conditions used in vitro were consistent with those of the smoker.
Firstly, the researcher evaluated diverse smoke toxicity from conventional cigarettes and the volatile component with no nicotine. Therefore, they assess that acute toxicity is mainly induced (about 80%) by the volatile components rather than nicotine. Secondly, researchers exposed different cell cultures to the same amount of nicotine from different products (classic cigarettes, electronic cigarettes) to demonstrate no toxicity associated with the aerosol of electronic cigarettes.
Scientists performed a further comparison between the aerosol from e-cigarettes and heated tobacco products showing no cytotoxic effect on bronchial cells on both products.
“This is a fundamental step for the international research on the electronic cigarettes,” stressed prof. Massimo Caruso, CoEHAR researcher involved in the Replica project. “In doing so, we developed shared research standards replicable in the future to avoid the proliferation of scientific studies with inaccurate and ineffective methodologies.”
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